Biochemistry Lab 5

Lipids (2)

http://www.mediafire.com/?j4lp1y9mgtbz944

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test for unsaturated fat

http://www.youtube.com/watch?v=2C_6ax2TsV8

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Soap (1ml) + H₂O  (1ml) and well shake → Miscible in water

Soap (1ml) + conc. H₂SO₄  (1ml) and well shake   → The oil or fat is Separated at the surface

Soap (1ml) +  NaCl (1ml) and well shake → The conc. Soap is Separated at the surface white ppt. is formed and insoluble in water .

Soap (1ml) + 10% CaCl₂ (1ml) and well shake → White ppt. is formed and insoluble in water