Biochemistry Lab 7

Vitamin C

http://www.mediafire.com/?r75xhcf33t5dq6e



Biochemistry Lab 6

DNA extraction

licture

http://www.mediafire.com/?j1wy6jyc545c2f5

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http://www.youtube.com/watch?v=X6kEWPI5-S4&feature=related

http://www.youtube.com/watch?v=UlzERP3KGZ0

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Step 1 : Breaking down cell membrane :

Step 2 : Heating and denaturing proteins in 15 minutes :

Step 3 : Precipitating DNA from alcohol solution :

Remove the beaker and place it in an ice bath for 10 minutes

The whitish viscous strands of DNA should precipitate out of the alcohol layer and form a whitish interface

Putting the thread DNA on the slid

Biochemistry Lab 5

Lipids (2)

http://www.mediafire.com/?j4lp1y9mgtbz944

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test for unsaturated fat

http://www.youtube.com/watch?v=2C_6ax2TsV8

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Soap (1ml) + H₂O  (1ml) and well shake → Miscible in water

Soap (1ml) + conc. H₂SO₄  (1ml) and well shake   → The oil or fat is Separated at the surface

Soap (1ml) +  NaCl (1ml) and well shake → The conc. Soap is Separated at the surface white ppt. is formed and insoluble in water .

Soap (1ml) + 10% CaCl₂ (1ml) and well shake → White ppt. is formed and insoluble in water