Thursday, 21 April 2011

Biochemistry Lab 6

DNA extraction
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http://www.youtube.com/watch?v=X6kEWPI5-S4&feature=related


http://www.youtube.com/watch?v=UlzERP3KGZ0

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Step 1 : Breaking down cell membrane :




Step 2 : Heating and denaturing proteins in 15 minutes :



Step 3 : Precipitating DNA from alcohol solution :
Remove the beaker and place it in an ice bath for 10 minutes


The whitish viscous strands of DNA should precipitate out of the alcohol layer and form a whitish interface


Putting the thread DNA on the slid


Wednesday, 6 April 2011

Biochemistry Lab 5

Lipids (2)




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test for unsaturated fat

http://www.youtube.com/watch?v=2C_6ax2TsV8

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Soap (1ml) + H2O  (1ml) and well shake → Miscible in water



Soap (1ml) + conc. H2SO4  (1ml) and well shake   The oil or fat is Separated at the surface


Soap (1ml) +  NaCl (1ml) and well shake → The conc. Soap is Separated at the surface white ppt. is formed and insoluble in water .




Soap (1ml) + 10% CaCl2 (1ml) and well shake → White ppt. is formed and insoluble in water

Friday, 25 March 2011

Biochemistry Lab 4

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تجربه saponification



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Make the soap
( in our lap )


بصيغه الـ  MP4
بصيغه الـ AVI

Thursday, 3 March 2011

Tuesday, 22 February 2011

biochemistry Lab 1




biochemistry Lab 1(Note)
__________
video of
amylase activity test
__________

 video of
Benedict's test